genesig Easy DNA/RNA Extraction Kit

SKU: Z-genesigEASY-EK

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Product Description
Ordering
Resources

Information

The genesig easy DNA/RNA extraction protocol begins with a simple lysis step where cells and tissue are lysed to release their nucleic acid. Then minute magnetic particles are added to bind to DNA/RNA. When placed on to the genesig magnetic separator the particles are pulled to the side of the tube making it easy to remove the unwanted supernatant with a pipette. Then a series of simple wash steps are performed before the DNA/RNA is washed off the beads back in to solution, ready for analysis by real-time PCR.

Its fast, and incredibly easy to perform.

Suitable sample types:

Clinical and Veterinary - including whole blood, plasma, and serum; saliva and sputum; faeces and urine; tissues; dry swabs; and bacterial culture broth
Food - including meat, fish, milk, and cooked or processed food products
Environmental - including plant, soil, and water

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Product Features

Product features

Extracts DNA and RNA with high yields in 45-60 mins
Works with huge range of sample types
Safe protocol with no phenolic chemicals
No centrifuge required - for use in the lab or in the field

Ordering

We work hard to keep shipping costs to a minimum. Kits are normally sent by courier to ensure rapid delivery. Cost varies according to your location. If you are using the online shopping facility this will be calculated for you automatically, otherwise please contact us for detailed pricing.

We sell our products across the globe. You will either have the option to purchase through us directly or through a trusted distributor, depending on your location.

Please enquire for the alternative Standard, Advanced, or Easy Kit formats if not listed. Kit formats not listed will be made to order and typically have a dispatch time of 4-6 weeks.

Resources

genesig Easy extraction kit Handbook

Extraction from tissue samples from the spleen, liver and brain

Alathari S, Chaput DL, Bolaños LM, Joseph A, Jackson VLN, Verner-Jeffreys D, Paley R, Tyler CR, Temperton B. A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia. Viruses. 2023 Apr 14;15(4):965. doi: 10.3390/v15040965. Erratum in: Viruses. 2023 Jun 29;15(7): PMID: 37112945; PMCID: PMC10145788.

A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia - PMC (nih.gov)

Extraction from whole blood (EDTA)

Gummery L, Jallow S, Raftery AG, Bennet E, Rodgers J, Sutton DGM. Comparison of loop-mediated isothermal amplification (LAMP) and PCR for the diagnosis of infection with Trypanosoma brucei ssp. in equids in The Gambia. PLoS One. 2020 Aug 24;15(8):e0237187. doi: 10.1371/journal.pone.0237187. PMID: 32833981; PMCID: PMC7444819.

Comparison of loop-mediated isothermal amplification (LAMP) and PCR for the diagnosis of infection with Trypanosoma brucei ssp. in equids in The Gambia - PMC (nih.gov)

Extracting from cell culture supernatant – Also using genesig C.trachomatis Advanced kit and PrecisionPLUS mastermix

Yang S, Traore Y, Jimenez C, Ho EA. Autophagy induction and PDGFR-β knockdown by siRNA-encapsulated nanoparticles reduce chlamydia trachomatis infection. Sci Rep. 2019 Feb 4;9(1):1306. doi: 10.1038/s41598-018-36601-y. PMID: 30718536; PMCID: PMC6361950.

Autophagy induction and PDGFR-β knockdown by siRNA-encapsulated nanoparticles reduce chlamydia trachomatis infection | Scientific Reports (nature.com)

Extracting RNA from fecal samples of sheep and goats

AlAzemi MS, Majeed QAH, Samy A, Henedi AA, Youssef W, Abdou NMI. Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait. Open Vet J. 2021 Jul-Sep;11(3):500-507. doi: 10.5455/OVJ.2021.v11.i3.23. Epub 2021 Sep 17. PMID: 34722215; PMCID: PMC8541720.

Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait - PMC (nih.gov)

Extraction from cultures, and fecal samples from pigs

Wu Y, Tian K, Zhang Y, Guo H, Li N, Wang Z, Zhao J. Rapid and visual detection of Lawsonia intracellularis with an improved recombinase polymerase amplification assay combined with a lateral flow dipstick. BMC Vet Res. 2019 Mar 21;15(1):97. doi: 10.1186/s12917-019-1841-9. PMID: 30898117; PMCID: PMC6429820.

Rapid and visual detection of Lawsonia intracellularis with an improved recombinase polymerase amplification assay combined with a lateral flow dipstick - PMC (nih.gov)

Extraction from bacterial cultures

Del Rio-Rodriguez RE, Ramirez-Paredes JG, Soto-Rodriguez SA, Shapira Y, Huchin-Cortes MDJ, Ruiz-Hernandez J, Gomez-Solano MI, Haydon DJ. First evidence of fish nocardiosis in Mexico caused by Nocardia seriolae in farmed red drum (Sciaenops ocellatus, Linnaeus). J Fish Dis. 2021 Aug;44(8):1117-1130. doi: 10.1111/jfd.13373. Epub 2021 Apr 13. PMID: 33848372.

First evidence of nocardiosis in farmed red drum (Sciaenops ocellatus, Linnaeus) caused by Nocardia seriolae in the Gulf of Mexico | bioRxiv

Extraction from pure cultures of P. acnes grown in BHI or THIO media

Neama Ahmad Alnabati, Ahmed Mahmoud Al-Hejin, Samah Omar Noor, Mohamed Morsi M. Ahmed, Mohamed Abu-Zeid & Nouf Talal Mleeh (2021) The antibacterial activity of four Saudi medicinal plants against clinical isolates of Propionibacterium acnes, Biotechnology & Biotechnological Equipment, 35:1, 415-424, DOI: 10.1080/13102818.2021.1885992

Full article: The antibacterial activity of four Saudi medicinal plants against clinical isolates of Propionibacterium acnes (tandfonline.com)

Comparison of four commercial DNA extraction kits for the recovery of Bacillus spp. spore DNA from spiked powder samples - ScienceDirect

genesig Easy DNA/RNA Extraction Kit

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