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Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Product features
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Advanced kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control - Read through VIC channel* (150 tests)
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Standard kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
RNAse/DNAse free water
DNA amplification was carried out using Primerdesign™ Ltd., and T. gondii repeat region genesig® Standard kit and oasig™ lyophilized 2× qPCR Mastermix (Uk) qPCR were performed for DNA amplification in final volume 20 μl. The amplification was performed in Mx3000P platforms.
Saad NM, Hussein AAA, Ewida RM. Occurrence of Toxoplasma gondii in raw goat, sheep, and camel milk in Upper Egypt. Vet World. 2018 Sep;11(9):1262-1265. doi: 10.14202/vetworld.2018.1262-1265. Epub 2018 Sep 15. PMID: 30410231; PMCID: PMC6200564.
Occurrence of Toxoplasma gondii in raw goat, sheep, and camel milk in Upper Egypt - PMC (nih.gov)
Purified DNA was then tested for the presence of Chlamydia psittaci (Primerdesign Ltd Chlamydia psittaci, gidA gene—genesig Advanced Kit, Rownhams, UK), Listeria spp. (Primerdesign Ltd Lysteria, Invasion-associated Protein p60 (iap) gene—genesig Advanced Kit, Rownhams, UK), Neospora caninum (Primerdesign Ltd Neospora caninum, Nc5 marker genomic sequence—genesig Advanced Kit, Rownhams, UK), and Toxoplasma gondii (Primerdesign Ltd Toxoplasma gondii, Repeat region—genesig Advanced Kit, Rownhams, UK).
Zaccaria G, Lorusso A, Hierweger MM, Malatesta D, Defourny SV, Ruggeri F, Cammà C, Ricci P, Domenico MD, Rinaldi A, Decaro N, D'Alterio N, Petrini A, Seuberlich T, Marcacci M. Detection of Astrovirus in a Cow with Neurological Signs by Nanopore Technology, Italy. Viruses. 2020 May 11;12(5):530. doi: 10.3390/v12050530. PMID: 32403368; PMCID: PMC7290991.
Molecular detection of abortive agents (Brucella spp, T. gondii, N. caninum, Schmallenberg virus, Leptospira spp. Chlamydiacae, BDV and BTV) was carried out on the samples taken from the fetus and two lambs with neurological symptoms. DNA extraction was executed with a Maxwell® 16 Cell and Tissue DNA Purification Kit (Promega Italia Srl, Milan, Italy). The DNA purity was checked by NanoDrop2000 (ThermoFisher Scientific, Waltham, MA, USA). The mix was prepared using the Genesig Advanced Kit (Genesig, York House, School Lane, Chandler’s Ford, UK), according to the manufacturer’s instructions, and Real-Time PCR was performed (QuantStudio™ 7 Pro Applied Biosystems, CA, USA).
De Angelis ME, Martino C, Chiaverini A, Di Pancrazio C, Di Marzio V, Bosica S, Malatesta D, Salucci S, Sulli N, Acciari VA, Pomilio F. Co-Infection of L. monocytogenes and Toxoplasma gondii in a Sheep Flock Causing Abortion and Lamb Deaths. Microorganisms. 2022 Aug 15;10(8):1647. doi: 10.3390/microorganisms10081647. PMID: 36014064; PMCID: PMC9415574.
quantitative PCR was performed on CSF samples for the detection of Neospora caninum and Toxoplasma gondii using N caninum Nc5 marker genomic sequence assays and Genesig T gondii repeat region, respectively. Assays were performed by Langford Vets Diagnostic Lab on a Qtower3 (Analytik Jena, Germany) following a laboratory‐validated thermocycling protocol.
Jones BS, Harcourt-Brown T. Comparison of serum creatine kinase and aspartate aminotransferase activity in dogs with Neospora meningoencephalitis and noninfectious meningoencephalitis. J Vet Intern Med. 2022 Jan;36(1):141-145. doi: 10.1111/jvim.16334. Epub 2021 Dec 3. PMID: 34859908; PMCID: PMC8783338.
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