Herpes simplex Type 1 and 2 (HHV1&2)

Kit

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Product Description
Ordering
Resources

Information

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. PrimerDesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Product Features

Product features

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings

genesig® kits are sold for research use only and are not licensed for diagnostic procedures.

Advanced kit contents:

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control - Read through VIC channel* (150 tests)
Endogenous control (150 tests)
RNAse/DNAse free water

*alternative fluorophores available on request

Standard kit contents:

Ordering

We work hard to keep shipping costs to a minimum. Kits are normally sent by courier to ensure rapid delivery. Cost varies according to your location. If you are using the online shopping facility this will be calculated for you automatically, otherwise please contact us for detailed pricing.

We sell our products across the globe. You will either have the option to purchase through us directly or through a trusted distributor, depending on your location.

Please enquire for the alternative Standard, Advanced, or Easy Kit formats if not listed. Kit formats not listed will be made to order and typically have a dispatch time of 4-6 weeks.

Resources

Advanced Kit Handbook Easy Kit Handbook Standard Kit Handbook

The human DNA herpesviruses, including Epstein-Barr virus (EBV), and herpes simplex type 1 (HSV-1) were quantified using a qRT-PCR method with the genesig Standard Kits (Primerdesign Ltd.) as described above.

Paradowska, E., Jabłońska, A., Studzińska, M. et al. Detection and genotyping of CMV and HPV in tumors and fallopian tubes from epithelial ovarian cancer patients. Sci Rep 9, 19935 (2019).

Detection and genotyping of CMV and HPV in tumors and fallopian tubes from epithelial ovarian cancer patients (nih.gov)

The presence of HHV1 and HHV2, HHV4–5 and BK, HHV6, HHV7, HHV8, JCV and SV40 viruses was detected using dedicated Genesig Advanced kits (Primerdesign Ltd., York House, School Lane, Chandler's Ford, United Kingdom) by qPCR, following the instructions of the manufacturer on the ABI7500 fast (Applied Biosystem, MA, USA).

Arsene DE, Milanesi E, Dobre M. Viral oncogenesis in tumours of the central nervous system: reality or random association? A retrospective study on archived material. J Cell Mol Med. 2022 Mar;26(5):1413-1420. doi: 10.1111/jcmm.17064. Epub 2022 Feb 2. PMID: 35112466; PMCID: PMC8899179.

Viral oncogenesis in tumours of the central nervous system: reality or random association? A retrospective study on archived material - PMC (nih.gov)

Viral load quantification of herpes simplex virus (HSV)-1, HSV-2, HHV-3, EBV, CMV, HHV-7, and HHV-8 was performed by reverse transcription-polymerase chain reaction (PCR) using the genesig quantitative PCR (qPCR) detection kit (PrimerDesign Ltd).

Osborne BJW, Marsh AK, Huibner S, Shahabi K, Liu C, Contente T, Nagelkerke NJD, Kovacs C, Benko E, Price L, MacDonald KS, Kaul R. Clinical and Mucosal Immune Correlates of HIV-1 Semen Levels in Antiretroviral-Naive Men. Open Forum Infect Dis. 2017 Feb 21;4(2):ofx033. doi: 10.1093/ofid/ofx033. PMID: 28534034; PMCID: PMC5421353.

Clinical and Mucosal Immune Correlates of HIV-1 Semen Levels in Antiretroviral-Naive Men - PMC (nih.gov)

Samples were also tested, and genome numbers were determined using validated standards provided by the Path-HSV1-genesig real-time PCR detection kit for human herpesvirus 1 (herpes simplex virus 1) (PrimerDesign, Ltd., South Hampton, United Kingdom).

Chouljenko DV, Kim IJ, Chouljenko VN, Subramanian R, Walker JD, Kousoulas KG. Functional hierarchy of herpes simplex virus 1 viral glycoproteins in cytoplasmic virion envelopment and egress. J Virol. 2012 Apr;86(8):4262-70. doi: 10.1128/JVI.06766-11. Epub 2012 Feb 8. PMID: 22318149; PMCID: PMC3318672.

Functional Hierarchy of Herpes Simplex Virus 1 Viral Glycoproteins in Cytoplasmic Virion Envelopment and Egress - PMC (nih.gov)

Samples were also tested and genome numbers were determined using validated standards provided by the Path-HSV-1-genesig real-time PCR detection kit for human herpesvirus 1 (herpes simplex type 1) (PrimerDesign, Ltd., South Hampton, United Kingdom).

David AT, Saied A, Charles A, Subramanian R, Chouljenko VN, Kousoulas KG. A herpes simplex virus 1 (McKrae) mutant lacking the glycoprotein K gene is unable to infect via neuronal axons and egress from neuronal cell bodies. mBio. 2012 Jul 24;3(4):e00144-12. doi: 10.1128/mBio.00144-12. PMID: 22829677; PMCID: PMC3413403.

A Herpes Simplex Virus 1 (McKrae) Mutant Lacking the Glycoprotein K Gene Is Unable To Infect via Neuronal Axons and Egress from Neuronal Cell Bodies - PMC (nih.gov)

Herpes simplex Type 1 and 2 (HHV1&2)

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