Hepatitis Delta Virus

Kit

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Product Description
Ordering
Resources

Information

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Product Features

Product features

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings

genesig® kits are sold for research use only and are not licensed for diagnostic procedures.

Advanced kit contents:

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control - Read through VIC channel* (150 tests)
Endogenous control (150 tests)
RNAse/DNAse free water

*alternative fluorophores available on request

Standard kit contents:

Ordering

We work hard to keep shipping costs to a minimum. Kits are normally sent by courier to ensure rapid delivery. Cost varies according to your location. If you are using the online shopping facility this will be calculated for you automatically, otherwise please contact us for detailed pricing.

We sell our products across the globe. You will either have the option to purchase through us directly or through a trusted distributor, depending on your location.

Please enquire for the alternative Standard, Advanced, or Easy Kit formats if not listed. Kit formats not listed will be made to order and typically have a dispatch time of 4-6 weeks.

Resources

Advanced Kit Handbook Easy Kit Handbook Standard Kit Handbook

HAV (target/5′ NCR), HCV (5′UTR), HEV (ORF2), HIV-1 (target/POL), HIV-2 (target/POL), HTLVI (target/POL), HTLVII (target/POL), and WNV (5′UTR) positive specimens were quantified using the Primer Design Genesig kit (Primerdesign Ltd, United Kingdom) according to the manufacturer’s protocol (OneStep RT-qPCR protocol). Each kit contained a positive control template for the PCR set up and for copy number determination (generated serial dilutions for the standard curve). The RT-qPCR assays were performed on a ViiA7 Applied Biosystems real-time PCR system.

De Giorgi V, Zhou H, Alter HJ, Allison RD. A microarray-based pathogen chip for simultaneous molecular detection of transfusion-transmitted infectious agents. J Transl Med. 2019 May 14;17(1):156. doi: 10.1186/s12967-019-1905-4. PMID: 31088488; PMCID: PMC6518760.

A microarray-based pathogen chip for simultaneous molecular detection of transfusion–transmitted infectious agents - PMC (nih.gov)

HAV RNA sequence detection by a Genesig HAV Real-Time PCR kit. The sequence selected is proprietary but HAV specific.

Franklin N, Camphor H, Wright R, Stafford R, Glasgow K, Sheppeard V. Outbreak of hepatitis A genotype IB in Australia associated with imported frozen pomegranate arils. Epidemiol Infect. 2019 Jan;147:e74. doi: 10.1017/S0950268818003515. PMID: 30869018; PMCID: PMC6518746.

Outbreak of hepatitis A genotype IB in Australia associated with imported frozen pomegranate arils - PMC (nih.gov)

HDV viral load was determined with Primerdesign HDV genesig assay (Primerdesign Ltd, United Kingdom) which is characterized by high priming efficiencies of >95% and can detect less than 100 copies of target template and was validated with an external control program (QCMD HDV14, QCMD, Glasgow, Scotland).

Shirazi R, Ram D, Rakovsky A, Bucris E, Gozlan Y, Lustig Y, Shaked-Mishan P, Picard O, Shemer-Avni Y, Ben-Zvi H, Halutz O, Lurie Y, Veizman E, Carlebach M, Braun M, Naftaly MC, Shlomai A, Safadi R, Mendelson E, Sklan EH, Ben-Ari Z, Mor O. Characterization of hepatitis B and delta coinfection in Israel. BMC Infect Dis. 2018 Feb 27;18(1):97. doi: 10.1186/s12879-018-3008-x. PMID: 29486716; PMCID: PMC6389180.

Characterization of hepatitis B and delta coinfection in Israel - PMC (nih.gov)

Qualitative HDV RNA PCR was done by reverse transcription-polymerase chain reaction (RT-PCR) using Primer Design™ genesig Kit for Hepatitis D Virus (Primerdesign, UK).

Sayad B, Naderi Y, Alavian SM, Najafi F, Janbakhsh A, Mansouri F, Vaziri S, Afsharian M, Norooznezhad F. Hepatitis D virus infection in Kermanshah, west of Iran: seroprevalence and viremic infections. Gastroenterol Hepatol Bed Bench. 2018 Spring;11(2):145-152. PMID: 29910856; PMCID: PMC5990919.

Hepatitis D virus infection in Kermanshah, west of Iran: seroprevalence and viremic infections - PMC (nih.gov)

Hepatitis Delta Virus

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