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Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Product features
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Advanced kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control - Read through VIC channel* (150 tests)
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Standard kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
RNAse/DNAse free water
An HCV Genesig® Standard Kit was employed to perform real-time PCR measuring 5’ untranslated region of HCV genome.
Wang, L., Cao, D., Wang, L. et al. HCV-associated exosomes promote myeloid-derived suppressor cell expansion via inhibiting miR-124 to regulate T follicular cell differentiation and function. Cell Discov 4, 51 (2018).
HCV detection results were reported by using UBI HCV EIA (Organon Teknika, Netherlands), and HCV detection One-step commercial kits (Genesig, Primerdesign, UK).
Safarnezhad Tameshkel F, Karbalaie Niya MH, Zamani F, Ajdarkosh H, Khoonsari M, Faraji AH, Motamed N, Nikkhah M, Ameli M, Miri SM, Azarkeivan A, Sohrabi MR, Keyvani H. Simultaneous Hepatitis C Virus Genotyping and Variant Detection in Patients with Thalassemia: A Single-Center Phylogenetic Study. Middle East J Dig Dis. 2022 Jan;14(1):124-130. doi: 10.34172/mejdd.2022.265. Epub 2022 Jan 30. PMID: 36619727; PMCID: PMC9489335.
Viral RNA was extracted from HCV-infected Huh-7 cells using the InvisorbSpin Virus RNA Mini Kit (Invitek, Hayward, CA, USA) according to the manufacturer’s protocol. The extracted RNA was stored at −80°C until further use. Viral nucleic acid was quantified using a viral nucleic acid detection kit (Genesig; PrimerDesign, Chandler’s Ford, UK).
Mahdy MM, El-Ekiaby NM, Hashish RM, Salah RA, Hanafi RS, Azzazy HM, Abdelaziz AI. miR-29a Promotes Lipid Droplet and Triglyceride Formation in HCV Infection by Inducing Expression of SREBP-1c and CAV1. J Clin Transl Hepatol. 2016 Dec 28;4(4):293-299. doi: 10.14218/JCTH.2016.00046. Epub 2016 Dec 26. PMID: 28097097; PMCID: PMC5225148.
The internal standard HCV RNA was synthesized by in vitro transcription (MEGAscript T7, Ambion, TX, USA) from pJFH-1 plasmid and quantified by a commercial kit (path-HCV, PrimerDesign, UK) at 1.1 × 1010 copies/ml.
Chen, J., Zhao, Y., Zhang, C. et al. Persistent hepatitis C virus infections and hepatopathological manifestations in immune-competent humanized mice. Cell Res 24, 1050–1066 (2014).
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