genesigPLEX qPCR Kit HBV/HCV/HIV1/HIV2

HIV-1

HAV (target/5′ NCR), HCV (5′UTR), HEV (ORF2), HIV-1 (target/POL), HIV-2 (target/POL), HTLVI (target/POL), HTLVII (target/POL), and WNV (5′UTR) positive specimens were quantified using the Primer Design Genesig kit (Primerdesign Ltd, United Kingdom) according to the manufacturer’s protocol (OneStep RT-qPCR protocol). 

De Giorgi V, Zhou H, Alter HJ, Allison RD. A microarray-based pathogen chip for simultaneous molecular detection of transfusion-transmitted infectious agents. J Transl Med. 2019 May 14;17(1):156. doi: 10.1186/s12967-019-1905-4. PMID: 31088488; PMCID: PMC6518760.

A microarray-based pathogen chip for simultaneous molecular detection of transfusion–transmitted infectious agents - PMC (nih.gov)

The HIV type 1 Genesig Standard kit (Primer design, Southampton, UK) was used to calibrate the viral titers.

Kim H, Choi MS, Inn KS, Kim BJ. Inhibition of HIV-1 reactivation by a telomerase-derived peptide in a HSP90-dependent manner. Sci Rep. 2016 Jul 1;6:28896. doi: 10.1038/srep28896. PMID: 27363520; PMCID: PMC4929463.

Inhibition of HIV-1 reactivation by a telomerase-derived peptide in a HSP90-dependent manner (nih.gov)

HIV-2

HAV (target/5′ NCR), HCV (5′UTR), HEV (ORF2), HIV-1 (target/POL), HIV-2 (target/POL), HTLVI (target/POL), HTLVII (target/POL), and WNV (5′UTR) positive specimens were quantified using the Primer Design Genesig kit (Primerdesign Ltd, United Kingdom) according to the manufacturer’s protocol (OneStep RT-qPCR protocol). 

De Giorgi V, Zhou H, Alter HJ, Allison RD. A microarray-based pathogen chip for simultaneous molecular detection of transfusion-transmitted infectious agents. J Transl Med. 2019 May 14;17(1):156. doi: 10.1186/s12967-019-1905-4. PMID: 31088488; PMCID: PMC6518760.

A microarray-based pathogen chip for simultaneous molecular detection of transfusion–transmitted infectious agents - PMC (nih.gov)

he Genesig Advanced Real-Time PCR HIV-2 Detection kit (Primer Design LTD, Southampton, UK) assay was performed as previously described (31), except for the use of the NIH-Z viral stock instead of the HIV-2 DNA calibrator included in the kit.

Jagodzinski LL, Manak MM, Hack HR, Liu Y, Peel SA. Performance evaluation of a laboratory developed PCR test for quantitation of HIV-2 viral RNA. PLoS One. 2020 Feb 28;15(2):e0229424. doi: 10.1371/journal.pone.0229424. PMID: 32109949; PMCID: PMC7048284.

Performance evaluation of a laboratory developed PCR test for quantitation of HIV-2 viral RNA - PMC (nih.gov)

For HIV-2 RNA, the first commercial test used by our laboratory was HIV-2 Real-Time RT-PCR Kit, followed from 2014 by Human Immunodeficiency Virus type 2 genesig Standard Kit.

Fumarola B, Calza S, Renzetti S, El Hamad I, Pezzoli MC, Izzo I, Degli Antoni M, Chiesa A, De Francesco M, Quiros-Roldan E, Caruso A, Castelli F, Focà E. Immunological Evolution of a Cohort of HIV-2 Infected Patients: Peculiarities of an Underestimated Infection. Mediterr J Hematol Infect Dis. 2022 Mar 1;14(1):e2022016. doi: 10.4084/mjhid.2022.016. PMID: 35444775; PMCID: PMC8992640.

Immunological Evolution of a Cohort of HIV-2 Infected Patients: Peculiarities of an Underestimated Infection - PMC (nih.gov)

The in-house HIV-2 nested PCR assay was evaluated using the four known HIV-2 antibody positive specimens. These specimens were also processed at a private laboratory using a commercial real-time HIV-2 PCR assay (Genesig Standard kit) that targets the integrase (pol) gene region.

Mafuyeka RT, Webber LM, Becker P, Mayaphi SH. HIV-1/2 differentiation in a South African public laboratory. South Afr J HIV Med. 2021 Mar 12;22(1):1185. doi: 10.4102/sajhivmed.v22i1.1185. PMID: 33824732; PMCID: PMC8008004.

HIV-1/2 differentiation in a South African public laboratory - PMC (nih.gov)