The BioBank is a high quality source of cDNA validated for use in real-time PCR experiments. The cDNA is reverse transcribed from high quality DNAse treated RNA, from a variety of tissues and cell cultures, using an optimised blend of oligo-dT and random nonamer primers. BioBank cDNA is therefore free of genomic DNA and PCR inhibitors and covers the widest possible range of RNA and mRNA transcripts in the specified tissue or cell line. Biobank cDNA is useful for expression profiling of newly identified genes, and also as a positive control for real-time PCR.
Positive control Primers
Positive control primer/probe are supplied with the kits which detect 18S ribosomal RNA. The 18S control primers are especially formulated to work under all recommended real-time PCR cycling conditions. i.e. Taqman cycling, PerfectProbe cycling, and SYBR green cycling conditions will all work perfectly with the kit.
The positive control primers will give a cycle threshold value of approximately CT=12 depending on the detection chemistry and the particular hardware settings in use.
Product features
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High quality cDNA
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Validated for use in real-time PCR
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Free from PCR inhibitors
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Guaranteed signal
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Reverse transcribed using optimal oligo-dT / random nonamer blend
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Kit contents
250ng of gDNA-free cDNA (minimum 25 reactions), Positive control 18S primer and probe set, PCR grade water
sample data
Mouse BioBankTM Generic cDNA (pooled cell lines)
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mo-gen |
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Generic pooled cDNA and control primers 25 rxn |
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| mo-x5 |
Any 5 cell or tissue cDNA samples 5 X 25 rxn |
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| mo-x10 |
Any 10 cell or tissue cDNA samples 10 X 25 rxn |
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