Human BioBank^TM cDNA for Real-Time PCR

The BioBank is a high quality source of cDNA validated for use in real-time PCR experiments. The cDNA is reverse transcribed from high quality DNAse treated RNA, from a variety of tissues and cell cultures, using an optimised blend of oligo-dT and random nonamer primers. BioBank cDNA is therefore free of genomic DNA and PCR inhibitors and covers the widest possible range of RNA and mRNA transcripts in the specified tissue or cell line. Biobank cDNA is useful for expression profiling of newly identified genes, and also as a positive control for real-time PCR.

Positive control Primers

Positive control primer/probe are supplied with the kits which detect 18S ribosomal RNA. The 18S control primers are especially formulated to work under all recommended real-time PCR cycling conditions. i.e. Taqman cycling, PerfectProbe cycling, and SYBR green cycling conditions will all work perfectly with the kit.

The positive control primers will give a cycle threshold value of approximately CT=12 depending on the detection chemistry and the particular hardware settings in use.

Product features

High quality cDNA
Validated for use in Real time PCR
Free from PCR inhibitors
Guaranteed signal
Reverse transcribed using optimal oligo-dT / random nonamer blend

Kit contents
250ng of gDNA-free cDNA (minimum 25 reactions), Positive control 18S primer and probe set, PCR grade water

sample data

Human BioBank^TM Generic cDNA (pooled cell lines)
	hu-gen	Generic pooled cDNA and control primers 25 rxn	click
	hu-x5	Any 5 cell or tissue cDNA samples 5 X 25 rxn	click
	hu-x10	Any 10 cell or tissue cDNA samples 10 X 25 rxn	click

Human BioBank^TM Tissue cDNA

hu-hea	Heart cDNA and control primers 25 rxn	click
hu-lun	Lung cDNA and control primers 25 rxn	click
hu-liv	Liver cDNA and control primers 25 rxn	click
hu-spl	Spleen cDNA and control primers 25 rxn	click
hu-bra	Brain cDNA and control primers 25 rxn	click
hu-pro	Prostate cDNA and control primers 25 rxn	click
hu-bla	Bladder cDNA and control primers 25 rxn	click
hu-s.mus	skeletal muscle cDNA and control primers 25 rxn	click
hu-s.int	Small intestine cDNA and control primers 25 rxn	click
hu-col	Colon cDNA and control primers 25 rxn	click
hu-adi	Adipose cDNA and control primers 25 rxn	click
hu-cer	cervix cDNA and control primers 25 rxn	click
hu-oes	Oesophagus cDNA and control primers 25 rxn	click
hu-kid	Kidney cDNA and control primers 25 rxn	click
hu-ova	Ovary cDNA and control primers 25 rxn	click
hu-tes	Testes cDNA and control primers 25 rxn	click
hu-thym	Thymus cDNA and control primers 25 rxn	click
hu-thyr	Thyroid cDNA and control primers 25 rxn	click
hu-tra	Trachea cDNA and control primers 25 rxn	click

Human BioBank^TM cultured cell line derived cDNA

hu-U937	U937 cDNA and control primers 25 rxn	click
hu-HeLa	HeLa cDNA and control primers 25 rxn	click
hu-Caco2	CACO2 cDNA and control primers 25 rxn	click
hu-H292	H292 cDNA and control primers 25 rxn	click
hu-Raji	Raji cDNA and control primers 25 rxn	click
hu-RD	RD cDNA and control primers 25 rxn	click

Human BioBank^TM Primary Cell line derived cDNA

hu-fib	Lung fibroblast cDNA and control primers 25 rxn	soon
hu-Tcell	T-cells cDNA and control primers 25 rxn	click
hu-huvec	HUVEC cDNA and control primers 25 rxn	click
hu-epi	Lung epithelium cDNA and control primers 25 rxn	soon