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| geNorm kits - Pig (Sus scrofa) |
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geNorm housekeeping gene selection kits.
For accurate gene quantitation, it is essential to normalise Real-Time PCR data to a fixed reference; one that is not affected by your experimental conditions. There is increasing evidence that normalising to a single, randomly selected housekeeping gene e.g. Actin Beta, introduces large and variable errors into the analysis. geNorm is a system for selecting the best candidate reference gene for a given experimental scenario. To perform geNorm analysis the user measures the expression of 6 or 12 housekeeping genes in their own samples. The geNorm software provided with the kit ranks the housekeeping genes in order of stability of expression. The output graphs therefore simply identify the best housekeeping genes for that particular experiment.
Product features
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Identifies best housekeeping genes
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Improves accuracy of subsequent experiments
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Improves reproducibility of subsequent experiments
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Sufficient for 10 geNorm analyses
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Kit contents
Lyophilised primers for a choice of 6 housekeeping genes (200rxns each) see below, PCR grade water, Latest geNorm software.
Sus scrofa glucose phosphate isomerase (GPI), mRNA.
Sus scrofa hyaluronan synthase 2 (SHAS2), mRNA.
Sus scrofa poly-ubiquitin (UBC)mRNA, 3 end.
Sus scrofa beta actin (ACTB) gene, partial cds.
Sus scrofa ATP synthase, mitochondrial F0 complex, (ATP5G1), mRNA.
Sus scrofa glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA, complete
Sus scrofa eukaryotic elongation factor 1 gamma-like protein (eEF-1) mRNA
Sus scrofa peptidylprolyl isomerase A (cyclophilin A) (PPIA), mRNA.
Sus scrofa glutathione peroxidase 1 (GPX1), mRNA.
Sus scrofa succinate dehydrogenase complex subunit A (SDHA) mRNA, partial cds.
Housekeeping genes
Once the best housekeeping genes have been identified using the geNorm kit, we can save you money by supplying individual kits for these genes. You can find more details of these products by clicking here.
ORDER ONLINE, E:ORDERS@PRIMERDESIGN.CO.UK, T: +44 (0)2380 312777, F: +44 (0)8708 362 155
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ge-SY-6-ss |
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6 primer only real time PCR assays for pig
housekeeping genes plus geNorm software |
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The complete solution
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