geNorm housekeeping gene selection kits. For accurate gene quantitation, it is essential to normalise Real-Time PCR data to a fixed reference; one that is not affected by your experimental conditions. There is increasing evidence that normalising to a single, randomly selected housekeeping gene e.g. Actin Beta, introduces large and variable errors into the analysis. geNorm is a system for selecting the best candidate reference gene for a given experimental scenario. To perform geNorm analysis the user measures the expression of 6 or 12 housekeeping genes in their own samples. The geNorm software provided with the kit ranks the housekeeping genes in order of stability of expression. The output graphs therefore simply identify the best housekeeping genes for that particular experiment.geNorm analysis will improve the quality of your real time PCR data.More than 500 papers have cited the geNorm method.
Product features
Identifies best housekeeping genes
Improves accuracy of subsequent experiments
Improves reproducibility of subsequent experiments
Improves sensitivity of subsequent experiments
Sufficient for 10 geNorm analyses
Kit contents Lyophilised primers (and probe) for 6 or 12 housekeeping genes (200rxns each), PCR grade water, latest geNorm software.If your species is not already represented in our range, we can develop a new geNorm kit for you - just ask