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| geNorm kits - Homo sapiens. |
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geNorm housekeeping gene selection kits.
For accurate gene quantitation, it is essential to normalise Real-Time PCR data to a fixed reference; one that is not affected by your experimental conditions. There is increasing evidence that normalising to a single, randomly selected housekeeping gene introduces large and variable errors into the analysis. geNorm is a system for selecting the best candidate reference gene for a given experimental scenario. To perform geNorm analysis the user measures the expression of 6 or 12 housekeeping genes in their own samples. The geNorm software provided with the kit ranks the housekeeping genes in order of stability of expression. The output graphs therefore simply identify the best housekeeping genes for that particular experiment.
Product features
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Identifies best housekeeping genes
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Improves accuracy of subsequent experiments
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Improves reproducibility of subsequent experiments
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Sufficient for 10 geNorm analyses
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Kit contents
Lyophilised primers (and probe) for 6 or 12 housekeeping genes (200rxns each) see below, PCR grade water, Latest geNorm software.
Homo sapiens actin, beta (ACTB), mRNA.
Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mRNA.
Homo sapiens ubiquitin C (UBC), mRNA.
Homo sapiens beta-2-microglobulin (B2M), mRNA.
Homo sapiens phospholipase A2 (YWHAZ), mRNA.
Homo sapiens splicing factor 3a, subunit 1(SF3A1), mRNA.
Human 18S rRNA gene
Homo sapiens cytochrome c-1 (CYC1), mRNA.
Homo sapiens eukaryotic translation initiation factor 4A, isoform 2 (EIF4A2), mRNA.
Homo sapiens succinate dehydrogenase complex, (SDHA), mRNA.
Homo sapiens topoisomerase (DNA) I (TOP1), mRNA.
Homo sapiens ATP synthase, (ATP5B), mRNA.
Housekeeping genes
Once the best housekeeping genes have been identified using the geNorm kit, we can save you money by supplying individual kits for these genes. You can find more details of these products by clicking here.
ORDER ONLINE, E:ORDERS@PRIMERDESIGN.CO.UK, T: +44 (0)2380 312777, F: +44 (0)8708 362 155
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ge-PP-6-hu |
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6 primer and PerfectProbe real time PCR assays for human housekeeping genes plus geNorm software |
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| ge-PP-12-hu |
12 primer and PerfectProbe real time PCR assays for human housekeeping genes plus geNorm software |
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click |
The complete solution
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